Figure 2 of
Yan, Mol Vis 2006;
12:205-214.Figure 2 of
Yan, Mol Vis 2006;
12:205-214.
Figure 2. SDS-PAGE analysis of the effect of carnosine on fructose 6-phosphate- and ribose-induced modifications of αL-crystallin
Incubation of αL-crystallin with fructose 6-phosphate (F6P) and ribose over 15-25 days resulted in significant crosslinking. Carnosine appeared to have a protective effect on this crosslinking. A: 0 days. B: 15 days incubation. C: 25 days incubation. Lane 1: Molecular weight marker. Lane 2: Control αL-crystallin. Lane 3: αL-crystallin with 100 mM F6P. Lane 4: αL-crystallin with 100 mM F6P and 50 mM carnosine. Lane 5: αL-crystallin with 100 mM F6P and 100 mM carnosine. Lane 6: αL-crystallin with 100 mM ribose. Lane 7: αL-crystallin with 100 mM ribose and 50 mM carnosine. Lane 8: αL-crystallin with 100 mM ribose and 100 mM carnosine.
