Figure 7. Effect of TUDCA on rd10 mouse retinal morphology

Representative micrographs stained with toluidine blue showing significant preservation of photoreceptor nuclei in the outer nuclear layer (ONL) in TUDCA treated eyes (right) compared with vehicle treated (left). Counts of photoreceptor nuclei were significantly greater in eyes with TUDCA treatment (right; n=7) compared to vehicle treatment (left; n=9; 522±45 versus 355±43 ONL nuclei per 400 μm-wide retinal section; p=0.0077). Combined inner segment (IS) and outer segment (OS) length was similarly preserved (14.8±1.14 versus 8.12±2.03 μm; p=0.0222), with no change in thickness of inner nuclear layer (INL) or ganglion cell layer (GCL).