Figure 6 of Linberg, Mol Vis 2006; 12:1674-1686.


Figure 6. Confocal micrographs of triple labeled cat retina

Confocal images of retina triple labeled with antibodies against calretinin (red), neurofilament (blue) and VAMP2 (synaptobrevin, green). A: Normal cat retina. An A-type HC has pinkish primary dendrites that underlie the red processes of the distal outer plexiform layer (OPL). The antibody to the synaptic vesicle protein VAMP2 labels the terminals of both rods and cones forming a thick uninterrupted layer (*) overlying the labeled HC processes; it also labels synaptic terminals in the inner plexiform layer (IPL). No HC processes extend beyond the boundaries of the OPL. Anti-calretinin-positive fibers (arrow) lie among anti-neurofilament-positive fibers running in bundles in the nerve fiber layer (NFL) in this section from central retina. ONL represents outer nuclear layer; INL represents inner nuclear layer; GCL represents ganglion cell layer. B: 28 day detached cat retina. The layer of labeled cone and rod terminals has thinned and is discontinuous (*) as shown by the VAMP2 labeling. Basal, perinuclear labeling by VAMP2 (arrowheads) can be seen in a number of rod nuclei in the proximal ONL. A short HC outgrowth (arrow) appears directed to a retracted rod terminal labeled by the VAMP2 antibody.

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Linberg, Mol Vis 2006; 12:1674-1686 <http://www.molvis.org/molvis/v12/a191/>
©2006 Molecular Vision <http://www.molvis.org/molvis/>
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