Figure 14. Wholemount of a 28 day detached retina labeled with the antibody to calretinin conjugated to donkey-α-rabbit AMCA

Wholemount of a 28 day detached retina, labeled with the antibody to calretinin conjugated to donkey-α-rabbit AMCA, that shows several 'directed' neurites rising into the outer nuclear layer (ONL) from a pair of B-type axon terminals (HBat1, HBat2) lying distal to an A-type perikaryon (HA) and its radiating dendritic trunks. The axons (ax1, ax2) and terminal processes of the B-type HCs stain more intensely than the A-type cell since the former has a greater affinity for the calretinin antibody than does the latter. This oblique projection was derived from a series of 160 0.5 μm thick optical sections captured by a Zeiss LSM 510 two-photon microscope and can be seen as a movie.

Note that the slide bar at the bottom of the quicktime movie can be used to manually control the flow of the movie. If you are unable to view the movie, a representative frame is included below.