Figure 3 of Piri, Mol Vis 2006; 12:1660-1673.


Figure 3. Semiquantitative RT-PCR validation of microarray data

A: The lanes with odd numbers contain PCR products obtained with RNA isolated from control retinas, and the lanes with even numbers are from experimental retinas. AW529036, BM389543, BF281839, AI112106, BQ200097, BF284887, and BG665267 are uncharacterized genes or ESTs. U78517 is the cAMP-regulated guanine nucleotide exchange factor II. NM_017134 is arginase 1. NM_031144 is β-actin. B: Spot-densitometry analysis of the differentially expressed gene fragments obtained by semiquantitative RT-PCR (sqRT-PCR). The amount of first strand cDNA used in this experiment was normalized based on IDVs (integrated density values) of the PCR product corresponding to β-actin mRNA.

(24 K)
(19 K)

Piri, Mol Vis 2006; 12:1660-1673 <http://www.molvis.org/molvis/v12/a190/>
©2006 Molecular Vision <http://www.molvis.org/molvis/>
ISSN 1090-0535