Table 2 of
Roberts, Mol Vis 2006;
12:177-183.Table 2 of
Roberts, Mol Vis 2006;
12:177-183.
Table 2. Mutation screening conditions
The table indicates the temperatures and flow rates used to optimally separate hetero- and homoduplexes by denaturing High Performance Liquid Chromatography for each fragment spanning the RP1 hotspot.
4F 4G 4H
--------------------- -------------------- -------------------
54 °C at 0.9 ml/min 54 °C at 0.9 ml/min 55 °C at 1.5 ml/min
56.1 °C at 1.5 ml/min 54.5 °C at 0.9 ml/min 56 °C at 1.5 ml/min
57.5 °C at 0.9 ml/min 57 °C at 1.5 ml/min
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