Figure 4 of
Kannan, Mol Vis 2006;
Figure 4. Effect of t-butylhydroperoxide on vascular endothelial growth factor-A gene and protein expression in ARPE-19 cells
Effect of t-butylhydroperoxide treatment on expression of vascular endothelial growth factor-A (VEGF-A) mRNA (A) and protein (B) in confluent, nonpolarized ARPE-19 cells. The mRNA and protein were analyzed by realtime PCR and immunoblot analysis, respectively, the latter under reducing condition. Data are mean±SEM from three experiments. tBH treatment caused a linear increase in gene expression with a maximum increase at 5 h. Asterisk indicates p<0.05 versus control. Recombinant VEGF-A was used as a positive control to identify the VEGF-A band in the western blot.