Figure 1. Expression of ZO-1 in polarized ARPE-19 cells grown in transwell filters in 1% fetal bovine serum containing medium

Cells were fixed in 2% paraformaldehyde, blocked in normal goat serum, and incubated with anti-zonula occludens (ZO-1) antibody overnight at 4 °C and mounted with DAPI. A: Control, untreated cells B: Cells treated with 150 μM t-butylhydroperoxide for 5 h.