Table 1 of Srivastava, Mol Vis 2006; 12:1615-1625.


Table 1.

Identification of spots excised from the normal human corneal epithelial cells following 2 D-gel electrophoresis and MALDI-TOF mass spectrometric analysis. The actual molecular weight (AMW) of proteins was obtained from the database in Swiss Prot (www.swissprot.com), and the observed molecular weight (OMW) was obtained from the mass spectrometric analysis data.

 Spot                                               AMW     OMW
number               Protein identified            (kDa)   (kDa)
------   ---------------------------------------   -----   -----
1        Similar to uracil-DNA glycosylase          14      22
2        Type II keratin subunit                    54      52
3        Human mitochondrial DNA binding protein    17      15
4        Ser/Thr protein phosphatase                55      51
5        Cyclophilin A                              17      16
6        Human galectin-7                           14      14
7        Keratin 19                                 44      44
8        Unidentified                               -       -
9        Keratin 19                                 44      44
10       alpha B-crystallin                         19      19
11-14    Unidentified                               -       -
15-19    Keratin 19                                 44      44
20       Unidentified                               -       -
21       Keratin 13                                 49      45
22       Unidentified                               -       -
23-26    HSP 27 kDa protein                         27      22
27       Golgi auotoantigen                         88      61
28-31    HSP 27 kDa protein                         27      22
32       Unidentified                               -       -
33       Keratin 13                                 49      45
34-36    Unidentified                               -       -
37       Lipoprotein gamma                           7       6
38       Annexin A2                                 38      38
39       Ribonuclear protein                        35      35
40       Annexin II                                 38      38
41       Keratin 2a                                 65      65
42       Hypothetic protein                         35      36
43       Annexin V                                  35      35
44-45    Actin                                      42      39
46-48    Keratin 19                                 44      44
49       ALDH                                       54      54
50       ALDH, NADP-Preferring                      50      50
51       beta-Actin                                 42      42
52-54    alpha-Enolase                              47      47
55       Actin                                      42      39
56       beta-Actin                                 41      42
57       Actin                                      42      39
58       Keratin 12                                 53      53
59       Unidentified                               -       -
60       H+ ATP synthase                            51      50
61       Keratin 12                                 53      53
62       Unidentified                               -       -
63       Keratin 8                                  53      53
64-65    Keratin 5                                  62      62
66       Keratin 3                                  64      64
67       Keratin 5                                  62      62
68       Keratin 12                                 53      53
69       Disulfide isomerase                        57      57
70       Unidentified                               -       -
71       Keratin 12                                 53      53
72       Lamin A/C                                  65      65
73       TRIM 29, isoform beta                      63      63
74       Unidentified                               -       -
75       Keratin 12                                 53      53
76       Lamin A                                    74      71
77       Keratin 12                                 53      53
78       78 kDa Glucose-regulated protein           78      72
79       Keratin 5                                  62      54
80       Molecular chaperone HSP A1L                70      69
81       Nerve growth factor, beta polypeptide      29      26

Srivastava, Mol Vis 2006; 12:1615-1625 <http://www.molvis.org/molvis/v12/a185/>
©2006 Molecular Vision <http://www.molvis.org/molvis/>
ISSN 1090-0535