Table 1 of
Zahir, Mol Vis 2006;
12:1606-1614.
Table 1. List of primary antibodies used for immunocytochemistry
The following primary antibodies were utilized for immunocytochemical analysis of late RPCs grown in the absence or presence of sorbitol.
Antibodies Type Specificity Source Dilution
---------- ----------------- ------------------- ---------- --------
CRALBP Mouse monoclonal RPE and Muller glia ABR 1:1000
GFAP Mouse monoclonal Astrocytes and Chemicon 1:400
Rabbit polyclonal Muller glia Dako 1:3000
Ki67 Mouse monoclonal Proliferating cells Novocastra 1:100
nestin Mouse monoclonal Neural progenitors DSHB 1:1
(Rat-401)
PKCα Mouse monoclonal Bipolar neurons Santa Cruz 1:200
recoverin Rabbit polyclonal Rod photoreceptors Chemicon 1:1000
and midget cone
bipolar neurons
vimentin Mouse polyclonal Astrocytes and Sigma 1:200
Goat polyclonal Muller glia Sigma 1:40
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