Figure 4 of Zahir, Mol Vis 2006; 12:1606-1614.


Figure 4. RT-PCR and immunoblot analysis of late RPCs grown in the absence or presence of sorbitol

RT-PCR (A) and Immunoblot (B) analysis of late RPCs grown in the absence or presence of sorbitol. Late RPCs grown in the absence of sorbitol expressed high levels of nestin (neuronal progenitor cell marker, A). After 7 days of sorbitol treatment there was down-regulation in nestin transcripts, along with a concomitant increase in CRALBP, GFAP, vimentin (glial cell markers), and PKCα (bipolar cell marker, A). Transcripts for recoverin (photoreceptor/cone bipolar cell marker) were not detected in untreated or treated RPCs (A). Immunoblot analysis of late RPCs, grown in the absence of sorbitol showed high levels of nestin expression (B). In addition, GFAP and PKCα protein expression was also detected in these cells at baseline levels (B). Treatment with sorbitol for 7 days resulted in an up-regulation of CRALBP. GFAP, vimentin and PKCα expression and a simultaneous down-regulation of nestin expression (B). However, recoverin expression was not detected in untreated or treated RPCs (B). Retinas from B6 mice (4 weeks) were used as negative or positive controls (B).

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Zahir, Mol Vis 2006; 12:1606-1614 <http://www.molvis.org/molvis/v12/a184/>
©2006 Molecular Vision <http://www.molvis.org/molvis/>
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