Figure 5. Dot matrix comparisons showing the existence of a two-gene IRBP locus in zebrafish

A: Dot matrix of human protein against the entire zebrafish gene locus. B: Human protein against translated Gene 1. C: Human protein against Gene 2 protein. The Pustell dot matrix program in MacVector was used employing the pam250 weight matrix and a hash value of 2 in all three panels. Two genes exist in tandem. The genes are oriented head to tail, the genes are close together, the genes are different, with Gene 1 a single exon and three-Repeats long and Gene 2 having four exons, but just two repeats. The optimum alignments to the human IRBP amino acid sequence showed that Gene 1 encodes Repeats 1, 2, and 3, while Gene 2 encoded Repeats 1 and 4.