Figure 4 of
Nickerson, Mol Vis 2006;
12:1565-1585.Figure 4 of
Nickerson, Mol Vis 2006;
12:1565-1585.
Figure 4. Alignment and comparison of Donor and Acceptor sites from the teleost fish Gene 2
This is a comparison to the accepted consensus donor and acceptor sites from human genes. At the beginning and end of each intron, respectively, invariant GT and AG dinucleotides are located. Most nearby nucleotides match the consensus donor or acceptor motif. These alignments indicate that the splice sites are all strongly conserved in position and sequence, and they all appear to function correctly.
Donor Acceptor
Exon | Intron Intron | Exon
Exon 1/Intron A/Exon 2
zebrafish TCCTCCAATG GTAAGATTTCTTTTC AGAAACTTTAGGTTTTGAGACAAGCTTACGAATGTTCCTTTAACTTCCAG AATCCCACAC
tetraodon GCCCCCCATG GTGAGACCCATGAAT TATTTAGCCATTAAATAGACATAACAAATGTGTATATTCTTCTCCAACAG GATTATACCC
fugu GAACGCCCCA GTGCTGCCCCCTATG ATGGTATGCTTCCAGTCTACTGAAACAAAATGTGTGTTCTTCTCCAACAG GATTATAGCC
medaka CCCACCAATG GTACAACCCACTACA AAAACTGTTGTAATGGTTGAAATGCTGCTTTGGTTCTGCCGTCTCTGCAG GAATACACCC
Exon 2/Intron B/Exon 3
zebrafish TTGATCTGAG GTGATTATTCAATTA TGGCTATAATTTGATCTGACAACTGGTATTTTATTTTCGTTGATTTCTAG AAATAACATC
tetraodon TCGACCTCAG GTGGGTAATCCCGCT AAAAATATAGTAAAATGAATATATCCTTTTTTTGAATGATTATTTTGCAG GAACAATGTT
fugu TTGACCTCAG GTGGGTAATCCTGCT AAACAAAGATCTGTCTACAGCAAACTTTTGAGGGTTTTTTTTTATTGCAG GAACAATGTT
medaka TCGACCTCAG GTGGGATGACACCCT GATTGGGTTTCAAACTTTTCTTTCAATCTTTTCTGCTCCTGAATTTTCAG GAACAACGTC
Exon 3/Intron C/Exon 4
zebrafish CAGCTCACAG GTACATTATTGCTGT GATATATTTCATATTACAAAACTGATGCATTAAATTCCACTTTTCTCTAG GCAGGAGATA
tetraodon GAACTCACTG GTAAAGAGTTTGTTG CAGACGAGTTAAACGACTTTACCGTTCAGCCCATCCTCCGCAGGCGTGAG GTACGGCTCC
fugu GAACTCACCG GTAAATAAAGATTGT TGTGCGCTCTTGCGTTCTCCATATTAACGCTAACCCATCATGCCCCACAG GCGTGAGGTA
medaka GAGCTCACAG GTGGGAAGGAGAAAA TATCGAGCTTGAGGCTCTTTACACCGTCAAACATCTCTTCTCCTCTGCAG GTGAGAGGTA
Consensus ...CAR GTRAGT... ...YYYYYYYYYYYNYAG G
sequence
patterns
|