Figure 2 of
Nickerson, Mol Vis 2006;
12:1565-1585.Figure 2 of
Nickerson, Mol Vis 2006;
12:1565-1585.
Figure 2. Alignment and comparison of Donor and Acceptor sites from the tetrapods
This is a comparison to the accepted consensus donor and acceptor sites from human genes. All the introns contain invariant GT and AG dinucleotides at the beginning and end of the intron. Most nearby nucleotides closely match the consensus donor or acceptor motif. The lariat consensus sequence is detected in about half the introns within 50 nucleotides of the acceptor site, and all have several A's, which may function in the absence of a closely matching lariat sequence. These alignments indicate that the splice sites are all strongly conserved in position and sequence, and they all appear to function well.
Donor Acceptor
Exon | Intron Intron | Exon
Exon 1/Intron A/Exon 2
chimp GCCCATGCAG GTGAGACCCAAGAGA TAAAACTGGCTGCTCCTCCTGACACTGAGTAGGACCTCCACCTCTTACAG ATCCCTTCCC
human GCCCATGCAG GTGAGACCCAAGAGA TAAAACTGGCTGCTCCTCCTGACACTGAGTAGGACCTCCAACTCTTACAG ATCCCTTCCC
cow ACCCATGCAG GTGAGTCACAAGGGA CTTTCAAATCTTTCCTTTCCTGACATGGAGCTGACCTCCCACTCTTTCAG ATCCCTTCCC
dog GCCCATGCAG GTGAGACACGCGGTC CCTGTGCCCGGGCTGAGTGGAGCCCCGCAGCCCCTGCCCTGCTCTTGCAG ATTCCCTCCC
rat GCCCATGCAG GTAAGGTACAAGAGA CCATACCCACCCCACTCCCAGGCACTGCCTGACTCTTCCCTCTCTTCCAG ATTCCCTCCC
mouse GCCCATGCAG GTAAGGTACAACAGA CGGCCATCTGCTGCTGTCCTGATACTGAGCCAGGCTTCCCACTCTTGCAG ATTCCCTCCC
opposum ATGCAGGTGA GTCTCCAAAACAGCC GAAGGAATGCGTTCCATGACTTTTCCAACTTTAATTTGAATTTTTGTCAG CTTCCCTCTC
chicken GCCAAAACAG GTACAGTTTTTTTTT AGCTATCAGTGCCAGCAAATGATTTGTTTTGTTTTTCCAAACTCTTTCAG ATCCCTTCCC
Xen. Trop GCCAATGCAG GTATGTAAAGTTTTA TTTCTACCAGTTTTTACATGTGCTAAAAATCTGCTTCTCAATCCTTTTAG ATCCCATCAC
Exon 2/Intron B/Exon 3
chimp TCGACATGAG GTCAGTGGCCAGGGG GCCTCACTGTGAGCTCAGCCCCTGAACAGGCTCTGCTTCCCATCCTTCAG GTTCAACATC
human TCGACATGAG GTCAGTGGCCAGGGG GCCTCACTGTGAGCTCAGCCCCTGAACAGGCTCTGCTTCCCATCCTTCAG GTTCAACATC
cow TCGACATGAG GTCAGTGGGCCTGAG GCTTCCCTGTGGGTTCTGCCCCTTAGTGGGCAACTTTTCCCTTCCTTCAG GTTCAACATC
dog TCGACATGAG GTCAGAGTCAGGGGG GGCCGCCCCATCAGCCCCTGCCCCCAGCGGTCCCACTTCTCTCCCCGCAG GTTCAACATC
rat TCGACATGAG GTCAGTGGGCTAGAC TTGAGTGCCTCTCAGACCACCCTCAGAGTCATTGTCTCTGCCTCCCTTAG ATTTAACATC
mouse TCGACATGAG GTCAGTGGGCTGGAC CTCCCTGTGAGCTCAGACGCCCTCCGAGTCATTGGCTCTGCTTCCCTTAG GTTCAACCTT
opposum TTGATATGAG GTAAACATGGGAGGG TCAGGGAGCTTTAATTTGGTTTCTAATGCTCACTAGTTCATGCCCAACAG GTTCAACATA
chicken TAGACATGAG GTAAACCTTTGAATG AGCTATCAGTGCCAGCAAATGATTTGTTTTGTTTTTCCAAACTCTTTCAG ATCCCTTCCC
Xen. Trop TTGATATGAG GTAGGTTGTCATTTC TAATATAAAATAAGACTTATATTCAACAACATTTATTGTTTACATTTCAG ATTTAACATC
Exon 3/Intron C/Exon 4
chimp CAGGTTGTAG GTACGTGGAGAAGCT CTCCAGGGTCCTGACATGACCCCCATCCTGAAGGGCCTTCTGTCTTCCAG GTGAACGCTA
human CAGGTTGTAG GTACGTGGAGAAGCT CTCCAGGGTCCTGACATGACCCCCATCCTGAAGGGCCTTATGTCTTCCAG GTGAACGCTA
cow CAGCTTGAAG GTGCGTGCAGAAGGC CCCAGCGTGGGGATTTGACTCCCTATTTTGAAGGGTCTTGTATCTTGCAG GTGAACGCTA
dog GACTCTGTCA GTGAGCTCTGGACCC CAGGGGCCCCGGGGACTGCCAGCCGGCTTGAAGGGCCCTGTGTCTTGCAG GTGAGCGCTA
rat CAGCTCACAG GTACATGAAGCCTTC GGTGGGAGGGTATTCATTTGGCCCCATGCTGAATGGCCTCTGTCCTCCAG GTGAACGATA
mouse CAGCTCACAG GTACCTGAAGCCTTC AGGTGGCGGGGGCTGATTTGACCCCGTGCTGAATGGCCTCTGTCCTCCAG GTGAACGATA
opposum ACAGTCTCAG GTTGCAGGTGAGAGG CAATTACAGTCCTCCAGAATCTTATATTCTGTCAATTTTGTCTCTTGCAG GTGAACGATA
chicken TAGACATGAG GTAAACCTTTGAATG AATCTCTGAAAATGGTGAAATTTTGTGATGCTTTTTGTCCTTTACGGCAG GGGAGAGGTA
Xen. Trop CATCTTGTAG GTATGTAAGTGCACA TATTCATTTAAATTTATACCGCTGGATGTGCTTATTTTTTCCCTACACAG GAAATGCTTT
Consensus CAR GTRAGT... ...YYYYYYYYYYYNYAG G
|