Figure 3 of
Klintworth, Mol Vis 2006;
12:159-176.Figure 3 of
Klintworth, Mol Vis 2006;
12:159-176.
Figure 3. Alignment of CHST6 mutations with amino acid sequences of carbohydrate sulfotransferases
Shown here is the alignment of CHST6 mutations with amino acid sequences of human galactose/N-acetylgalactosamine/N-acetylglucosamine 6-O-sulfotransferase family of carbohydrate sulfotransferases (CHST1-7). In the figure, the red colored residues are identical to the consensus sequence and the blue colored residues are similar to the consensus sequence.The hydrophobic site, 5'-phosphate binding site, 3'-phosphate binding site and the N-glycosylation sites of CHST6 are also shown. The less than sign (<) indicates a deletion with frameshift, the greater than sign (>) indicates an insertion with frameshift, the sharp (#) indicates an insertion plus deletion with frameshift, the plus sign (+) indicates an in-frame insertion, and the plus/minus sign (±) indicates an in-frame insertion plus deletion.
CHST6 1 ------------------------------------------------------------
CHST5 1 ------------------------------------------------------------
CHST4 1 ------------------------------------------------------------
CHST7 1 -----------------------------------------------MKGRRRRRREYCK
CHST2 1 MSRSPQRALPPGALPRLLQAAPAAAPRALLPQWPRRPGRRWPASPLGMKVFRRKALVLCA
CHST1 1 ------------------------------------------------------------
CHST3 1 -------------------------------------MEKGLTLPQDCRDFVHSLKMRSK
hydrophobic site
______
> |
Changes ?XM << P
CHST6 1 ---------------------------------------------MWLPRVSSTAVTALL
CHST5 1 --------MGMRARVP----------------KVAHSTRRPPAARMWLPRFSSKTVTVLL
CHST4 1 --------------------------------------------MLLPKKMKLLLFLVSQ
CHST7 14 FALLLVLYTLVLLLVP----------------SVLDGGRDGDKGAEHCPGLQRSLGVWSL
CHST2 61 GYALLLVLTMLNLLDYKWHKEPLQQCNPDGPLGAAAGAAGGSWGRPGPPPAGPPRAHARL
CHST1 1 --------------------------------------------MQCSWKAVLLLALASI
CHST3 24 YALFLVFVVIVFVFIEKENKIISRVSDKLKQIPQALADANSTDPALILAENASLLSLSEL
hydrophobic site 5'-phosphate binding site
_______________________________________________ __
| |L
< C
Changes X R S< Y P CL
CHST6 16 LAQT------------------------FLLLFLVSRPGPSSPAGGEARVHVLVLSSWRS
CHST5 37 LAQTT-----------------------CLLLFIISRPGPSSPAGGEDRVHVLVLSSWRS
CHST4 17 MAILA-----------------------LFFHMYSHNISSLSMKAQPERMHVLVLSSWRS
CHST7 58 EAAAAGEREQGA-----EARAAEEGGANQSPRFPSNLSGAVGEAVSREKQHIYVHATWRT
CHST2 121 DLRTPYRPPAAA-----VGAAPAAAAGMAGVAAPPGNGTRGTGGVGDKRQLVYVFTTWRS
CHST1 17 AIQYTAIRTFTA-----KSFHTCPGLAEAGLAERLCEESPTFAYNLSRKTHILILATTRS
CHST3 84 DSAFSQLQSRLRNLSLQLGVEPAMEAAGEEEEEQRKEEEPPRPAVAGPRRHVLLMATTRT
5'-phosphate binding site
__
| <
LI T X S W G
Changes DLF R XP<T Q DVH SP MC X #LH PLD T
CHST6 52 GSSFVGQLFNQHPDVFYLMEPAWHVWTTLSQ--------GSAATLHMAVRDLVRSVFLCD
CHST5 74 GSSFLGQLFSQHPDVFYLMEPAWHVWTTLSQ--------GSAATLHMAVRDLMRSIFLCD
CHST4 54 GSSFVGQLFGQHPDVFYLMEPAWHVWMTFKQ--------STAWMLHMAVRDLIRAVFLCD
CHST7 113 GSSFLGELFNQHPDVFYLYEPMWHLWQALYP--------GSAVSLQGALRDMLRSLFRCD
CHST2 176 GSSFFGELFNQNPEVFFLYEPVWHVWQKLYP--------GDAVSLQGAARDMLSALYRCD
CHST1 72 GSSFVGQLFNQHLDVFYLFEPLYHVQNTLIPRFTQGKSPADRRVMLGASRDLLRSLYDCD
CHST3 144 GSSFVGEFFNQQGNIFYLFEPLWHIERTVSFE----PGGANAAGSALVYRDVLKQLFLCD
N-glycosylation site
___
| | > P
Changes V S C C LPX >CV L >X
CHST6 104 MDVFDAYLPWR-----------RNLSDLFQWAVSRALCSPPACSAFPRG----AISSEAV
CHST5 126 MDVFDAYMPQS-----------RNLSAFFNWATSRALCSPPACSAFPRG----TISKQDV
CHST4 106 MSVFDAYMEPGP----------RRQSSLFQWENSRALCSAPACDIIPQD----EIIPRAH
CHST7 165 FSVLRLYAPPGDPAARAPDTANLTTAALFRWRTNKVICSPPLCPGAPRARAEVGLVEDTA
CHST2 228 LSVFQLYSPAGSGGR------NLTTLGIFGAATNKVVCSSPLCPAYRKEVVG--LVDDRV
CHST1 132 LYFLENYIKPPPVN--------HTTDRIFRRGASRVLCSRPVCDPPGPAD---LVLEEGD
CHST3 200 LYVLEHFITPLP--------EDHLTQFMFRRGSSRSLCEDPVCTPFVKK-----VFEKYH
3'-phosphate binding site
______
| #
S C NQQT
Changes PX WPF R HC < >P±±±+E R SERLV
CHST6 149 CKP-LCARQSFTLAREACRSYSHVVLKEVRFFNLQVLYPLLSDPALNLRIVHLVRDPRAV
CHST5 171 CKT-LCTRQPFSLAREACRSYSHVVLKEVRFFNLQVLYPLLSDPALNLRIVHLVRDPRAV
CHST4 152 CRL-LCSQQPFEVVEKACRSYSHVVLKEVRFFNLQSLYPLLKDPSLNLHIVHLVRDPRAV
CHST7 225 CER-SCPPVAIRALEAECRKYPVVVIKDVRLLDLGVLVPLLRDPGLNLKVVQLFRDPRAV
CHST2 280 CK--KCPPQRLARFEEECRKYRTLVIKGVRVFDVAVLAPLLRDPALDLKVIHLVRDPRAV
CHST1 181 CVR-KCGLLNLTVAAEACRERSHVAIKTVRVPEVNDLRALVEDPRLNLKVIQLVRDPRGI
CHST3 247 CKNRRCGPLNVTLAAEACRRKEHMALKAVRIRQLEFLQPLAEDPRLDLRVIQLVRDPRAV
3'-phosphate binding site N-glycosylation site
__ _____________________________
|W Y | |
Changes FQ T +E D D X
CHST6 208 LRSREQTAKALARDNGIVLGTN--------------------------GTWVEADPGLRV
CHST5 230 LRSREAAGPILARDNGIVLGTN--------------------------GKWVEADPHLRL
CHST4 211 FRSRERTKGDLMIDSRIVMGQH--------------------------EQKLKKEDQPYY
CHST7 284 HNSRLKSRQGLLRESIQVLRTRQRGDRFHRVLLAHGVGARPGGQSRALPAAPRADFFLTG
CHST2 338 ASSRIRSRHGLIRESLQVVRSRDPRAHRMPFLEAAG-HKLG---AKKEGVGGPADYHALG
CHST1 240 LASRSETFRDTYRLWRLWYGTG---------------------------RK--PYNLDVT
CHST3 307 LASRMVAFAGKYKTWKKWLDDE---------------------------GQDGLREEEVQ
S
Changes W<RP C H K P
CHST6 242 VREVCRSHVRIAEAATLKPPPFLRGRYRLVRFEDLAREPLAEIRALYAFTGLSLTPQLEA
CHST5 264 IREVCRSHVRIAEAATLKPPPFLRGRYRLVRFEDLAREPLAEIRALYAFTGLTLTPQLEA
CHST4 245 VMQVICQSQLEIYKTIQSLPKALQERYLLVRYEDLARAPVAQTSRMYEFVGLEFLPHLQT
CHST7 344 ALEVICEAWLRDLLFARGAPAWLRRRYLRLRYEDLVRQPRAQLRRLLRFSGLRALAALDA
CHST2 394 AMEVICNSMAKTLQTALQPPDWLQGHYLVVRYEDLVGDPVKTLRRVYDFVGLLVSPEMEQ
CHST1 271 QLTTVCEDFSNSVSTGLMRPPWLKGKYMLVRYEDLARNPMKKTEEIYGFLGIPLDSHVAR
CHST3 340 RLRGNCESIRLSAELGLRQPAWLRGRYMLVRYEDVARGPLQKAREMYRFAGIPLTPQVED
N-glycosylation site N-glycosylation site
___ ___
| | | |
X Y
Changes X H C# X W <> D
CHST6 302 WIHNITHGSGPGARREAFKTSSRNALNVSQAWRHALPFAKIRRVQELCAGALQLLGYRPV
CHST5 324 WIHNITHGSGIGKPIEAFHTSSRNARNVSQAWRHALPFTKILRVQEVCAGALQLLGYRPV
CHST4 305 WVHNITRGKGMGD--HAFHTNARDALNVSQAWRWSLPYEKVSRLQKACGDAMNLLGYRHV
CHST7 404 FALNMTRGAAYGAD-RPFHLSARDAREAVHAWRERLSREQVRQVEAACAPAMRLLAYPRS
CHST2 454 FALNMTSGSGSSS--KPFVVSARNATQAANAWRTALTFQQIKQVEEFCYQPMAVLGYERV
CHST1 331 WIQNNTRGDPTLG--KHKYGTVRNSAATAEKWRFRLSYDIVAFAQNACQQVLAQLGYKIA
CHST3 400 WIQKNTQAAHDGS--G-IYSTQKNSSEQFEKWRFSMPFKLAQVVQAACGPAMRLFGYKLA
CHST6 362 YSEDEQRNLALDLVLPRGLNGFTWASSTASHPRN
CHST5 384 YSADQQRDLTLDLVLPRGPDHFSWASPD------
CHST4 363 RSEQEQRNLLLDLLS------T-WTVPEQIH---
CHST7 463 GEEGDAEQPREGETP------LEMDADGAT----
CHST2 512 NSPEEVKDLSKTLLR----------KPRL-----
CHST1 389 ASEEELKNPSVSLVE------ERDFRPFS-----
CHST3 457 RDAAALTNRSVSLLE------ERGTFWVT-----
|