Figure 3 of Klintworth, Mol Vis 2006; 12:159-176.


Figure 3. Alignment of CHST6 mutations with amino acid sequences of carbohydrate sulfotransferases

Shown here is the alignment of CHST6 mutations with amino acid sequences of human galactose/N-acetylgalactosamine/N-acetylglucosamine 6-O-sulfotransferase family of carbohydrate sulfotransferases (CHST1-7). In the figure, the red colored residues are identical to the consensus sequence and the blue colored residues are similar to the consensus sequence.The hydrophobic site, 5'-phosphate binding site, 3'-phosphate binding site and the N-glycosylation sites of CHST6 are also shown. The less than sign (<) indicates a deletion with frameshift, the greater than sign (>) indicates an insertion with frameshift, the sharp (#) indicates an insertion plus deletion with frameshift, the plus sign (+) indicates an in-frame insertion, and the plus/minus sign (±) indicates an in-frame insertion plus deletion.

CHST6     1 ------------------------------------------------------------
CHST5     1 ------------------------------------------------------------
CHST4     1 ------------------------------------------------------------
CHST7     1 -----------------------------------------------MKGRRRRRREYCK
CHST2     1 MSRSPQRALPPGALPRLLQAAPAAAPRALLPQWPRRPGRRWPASPLGMKVFRRKALVLCA
CHST1     1 ------------------------------------------------------------
CHST3     1 -------------------------------------MEKGLTLPQDCRDFVHSLKMRSK

                                                        hydrophobic site
                                                                  ______
                                                              >  |
Changes                                                  ?XM <<        P
CHST6     1 ---------------------------------------------MWLPRVSSTAVTALL
CHST5     1 --------MGMRARVP----------------KVAHSTRRPPAARMWLPRFSSKTVTVLL
CHST4     1 --------------------------------------------MLLPKKMKLLLFLVSQ
CHST7    14 FALLLVLYTLVLLLVP----------------SVLDGGRDGDKGAEHCPGLQRSLGVWSL
CHST2    61 GYALLLVLTMLNLLDYKWHKEPLQQCNPDGPLGAAAGAAGGSWGRPGPPPAGPPRAHARL
CHST1     1 --------------------------------------------MQCSWKAVLLLALASI
CHST3    24 YALFLVFVVIVFVFIEKENKIISRVSDKLKQIPQALADANSTDPALILAENASLLSLSEL

             hydrophobic site                  5'-phosphate binding site
_______________________________________________                       __
                                               |                     |L
              <                                                       C
Changes       X                           R        S<         Y   P   CL
CHST6    16 LAQT------------------------FLLLFLVSRPGPSSPAGGEARVHVLVLSSWRS
CHST5    37 LAQTT-----------------------CLLLFIISRPGPSSPAGGEDRVHVLVLSSWRS
CHST4    17 MAILA-----------------------LFFHMYSHNISSLSMKAQPERMHVLVLSSWRS
CHST7    58 EAAAAGEREQGA-----EARAAEEGGANQSPRFPSNLSGAVGEAVSREKQHIYVHATWRT
CHST2   121 DLRTPYRPPAAA-----VGAAPAAAAGMAGVAAPPGNGTRGTGGVGDKRQLVYVFTTWRS
CHST1    17 AIQYTAIRTFTA-----KSFHTCPGLAEAGLAERLCEESPTFAYNLSRKTHILILATTRS
CHST3    84 DSAFSQLQSRLRNLSLQLGVEPAMEAAGEEEEEQRKEEEPPRPAVAGPRRHVLLMATTRT

5'-phosphate binding site
           __
             |             <
                          LI     T   X                       S    W   G
Changes     DLF R XP<T Q  DVH   SP  MC    X                #LH   PLD  T
CHST6    52 GSSFVGQLFNQHPDVFYLMEPAWHVWTTLSQ--------GSAATLHMAVRDLVRSVFLCD
CHST5    74 GSSFLGQLFSQHPDVFYLMEPAWHVWTTLSQ--------GSAATLHMAVRDLMRSIFLCD
CHST4    54 GSSFVGQLFGQHPDVFYLMEPAWHVWMTFKQ--------STAWMLHMAVRDLIRAVFLCD
CHST7   113 GSSFLGELFNQHPDVFYLYEPMWHLWQALYP--------GSAVSLQGALRDMLRSLFRCD
CHST2   176 GSSFFGELFNQNPEVFFLYEPVWHVWQKLYP--------GDAVSLQGAARDMLSALYRCD
CHST1    72 GSSFVGQLFNQHLDVFYLFEPLYHVQNTLIPRFTQGKSPADRRVMLGASRDLLRSLYDCD
CHST3   144 GSSFVGEFFNQQGNIFYLFEPLWHIERTVSFE----PGGANAAGSALVYRDVLKQLFLCD

                           N-glycosylation site
                                   ___
                                  |   |  >        P
Changes     V  S  C   C                 LPX  >CV  L       >X
CHST6   104 MDVFDAYLPWR-----------RNLSDLFQWAVSRALCSPPACSAFPRG----AISSEAV
CHST5   126 MDVFDAYMPQS-----------RNLSAFFNWATSRALCSPPACSAFPRG----TISKQDV
CHST4   106 MSVFDAYMEPGP----------RRQSSLFQWENSRALCSAPACDIIPQD----EIIPRAH
CHST7   165 FSVLRLYAPPGDPAARAPDTANLTTAALFRWRTNKVICSPPLCPGAPRARAEVGLVEDTA
CHST2   228 LSVFQLYSPAGSGGR------NLTTLGIFGAATNKVVCSSPLCPAYRKEVVG--LVDDRV
CHST1   132 LYFLENYIKPPPVN--------HTTDRIFRRGASRVLCSRPVCDPPGPAD---LVLEEGD
CHST3   200 LYVLEHFITPLP--------EDHLTQFMFRRGSSRSLCEDPVCTPFVKK-----VFEKYH

                                               3'-phosphate binding site
                                                                  ______
                                                                 |   #
                             S           C                         NQQT
Changes         PX           WPF      R  HC   <         >P±±±+E R SERLV
CHST6   149 CKP-LCARQSFTLAREACRSYSHVVLKEVRFFNLQVLYPLLSDPALNLRIVHLVRDPRAV
CHST5   171 CKT-LCTRQPFSLAREACRSYSHVVLKEVRFFNLQVLYPLLSDPALNLRIVHLVRDPRAV
CHST4   152 CRL-LCSQQPFEVVEKACRSYSHVVLKEVRFFNLQSLYPLLKDPSLNLHIVHLVRDPRAV
CHST7   225 CER-SCPPVAIRALEAECRKYPVVVIKDVRLLDLGVLVPLLRDPGLNLKVVQLFRDPRAV
CHST2   280 CK--KCPPQRLARFEEECRKYRTLVIKGVRVFDVAVLAPLLRDPALDLKVIHLVRDPRAV
CHST1   181 CVR-KCGLLNLTVAAEACRERSHVAIKTVRVPEVNDLRALVEDPRLNLKVIQLVRDPRGI
CHST3   247 CKNRRCGPLNVTLAAEACRRKEHMALKAVRIRQLEFLQPLAEDPRLDLRVIQLVRDPRAV

3'-phosphate binding site                           N-glycosylation site
            __                   _____________________________
              |W         Y      |                             |
Changes       FQ     T  +E D    D                             X
CHST6   208 LRSREQTAKALARDNGIVLGTN--------------------------GTWVEADPGLRV
CHST5   230 LRSREAAGPILARDNGIVLGTN--------------------------GKWVEADPHLRL
CHST4   211 FRSRERTKGDLMIDSRIVMGQH--------------------------EQKLKKEDQPYY
CHST7   284 HNSRLKSRQGLLRESIQVLRTRQRGDRFHRVLLAHGVGARPGGQSRALPAAPRADFFLTG
CHST2   338 ASSRIRSRHGLIRESLQVVRSRDPRAHRMPFLEAAG-HKLG---AKKEGVGGPADYHALG
CHST1   240 LASRSETFRDTYRLWRLWYGTG---------------------------RK--PYNLDVT
CHST3   307 LASRMVAFAGKYKTWKKWLDDE---------------------------GQDGLREEEVQ

                                          S
Changes         W<RP                  C   H K P
CHST6   242 VREVCRSHVRIAEAATLKPPPFLRGRYRLVRFEDLAREPLAEIRALYAFTGLSLTPQLEA
CHST5   264 IREVCRSHVRIAEAATLKPPPFLRGRYRLVRFEDLAREPLAEIRALYAFTGLTLTPQLEA
CHST4   245 VMQVICQSQLEIYKTIQSLPKALQERYLLVRYEDLARAPVAQTSRMYEFVGLEFLPHLQT
CHST7   344 ALEVICEAWLRDLLFARGAPAWLRRRYLRLRYEDLVRQPRAQLRRLLRFSGLRALAALDA
CHST2   394 AMEVICNSMAKTLQTALQPPDWLQGHYLVVRYEDLVGDPVKTLRRVYDFVGLLVSPEMEQ
CHST1   271 QLTTVCEDFSNSVSTGLMRPPWLKGKYMLVRYEDLARNPMKKTEEIYGFLGIPLDSHVAR
CHST3   340 RLRGNCESIRLSAELGLRQPAWLRGRYMLVRYEDVARGPLQKAREMYRFAGIPLTPQVED

     N-glycosylation site     N-glycosylation site
               ___                    ___
              |   |                  |   |
                                         X                 Y
Changes            X                     H  C#           X W   <>   D
CHST6   302 WIHNITHGSGPGARREAFKTSSRNALNVSQAWRHALPFAKIRRVQELCAGALQLLGYRPV
CHST5   324 WIHNITHGSGIGKPIEAFHTSSRNARNVSQAWRHALPFTKILRVQEVCAGALQLLGYRPV
CHST4   305 WVHNITRGKGMGD--HAFHTNARDALNVSQAWRWSLPYEKVSRLQKACGDAMNLLGYRHV
CHST7   404 FALNMTRGAAYGAD-RPFHLSARDAREAVHAWRERLSREQVRQVEAACAPAMRLLAYPRS
CHST2   454 FALNMTSGSGSSS--KPFVVSARNATQAANAWRTALTFQQIKQVEEFCYQPMAVLGYERV
CHST1   331 WIQNNTRGDPTLG--KHKYGTVRNSAATAEKWRFRLSYDIVAFAQNACQQVLAQLGYKIA
CHST3   400 WIQKNTQAAHDGS--G-IYSTQKNSSEQFEKWRFSMPFKLAQVVQAACGPAMRLFGYKLA

CHST6   362 YSEDEQRNLALDLVLPRGLNGFTWASSTASHPRN
CHST5   384 YSADQQRDLTLDLVLPRGPDHFSWASPD------
CHST4   363 RSEQEQRNLLLDLLS------T-WTVPEQIH---
CHST7   463 GEEGDAEQPREGETP------LEMDADGAT----
CHST2   512 NSPEEVKDLSKTLLR----------KPRL-----
CHST1   389 ASEEELKNPSVSLVE------ERDFRPFS-----
CHST3   457 RDAAALTNRSVSLLE------ERGTFWVT-----

Klintworth, Mol Vis 2006; 12:159-176 <http://www.molvis.org/molvis/v12/a18/>
©2006 Molecular Vision <http://www.molvis.org/molvis/>
ISSN 1090-0535