Figure 1. Schematic illustration of polymerase chain reaction primers, anchors, and detection probes for the SAG gene

The probe for SAG11Flu is labeled with fluorescein at the 3' end and serves as an anchor probe for SAG11LCRed. The sensor probes, SAG11LCRed, is labeled with LCRed640 at the 5' end and spans the DNA region having the mutations. The region of the mutations of the SAG gene is shown below the illustration, and the mutation sites corresponding to 926delA are shown in bold letters and are indicated by arrows. Circle represents fluorescein; double circle represents LC-Red 640.