Table 1 of
Fernandes, Mol Vis 2006;
12:1526-1535.Table 1 of
Fernandes, Mol Vis 2006;
12:1526-1535.
Table 1. Primers for real-time reverse transcriptase-polymerase chain reaction and Northern blot
F represents forward frimer R represents reverse primer. RT-PCR is reverse transcriptase polymerase chain reaction.
GenBank
accesssion
Gene number Primers (5'- 3')
----- ---------- --------------------------------------------
RT-PCR
UbA52 BC061544 F: ctcttcaacgaggcggccgagcg
R: ttactgctccagtcgatggaagg
UbB BC060312 F: tggctattaattcttcagtctg
R: aacctttattaacatttttaac
UbC D17296 F: gaaggtcaaacaggaagata
R: gttaacagcttaaaagatagg
Actin NM_001101 F: aaggagaagctgtgctacgtcgccctgg
R: gatcttgatcttcattgtgctgggtgcc
Northern Blot
UbA52 NM_003333 F: tattgagccttctctccgccagc
R: tgctgctccagtcaatgaaagggacac
Ub BE298836 F: taactggtacccatatgcagatcttcgtgaaaacccttacc
R: actggtcggatcctcattcacgaagaactgcataaccacc
Actin NM_001101 F: aaggagaagctgtgctacgtcgccctgg
R: gatcttgatcttcattgtgctgggtgcc
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