Figure 6 of
Fernandes, Mol Vis 2006;
Figure 6. Cells exposed to oxidative stress show increased ability to conjugate exogenous ubiquitin to endogenous substrates
The ability of retinal endothelial cell lysates to conjugate radiolabeled exogenous exogenous 125I-ubiquitin to endogenous substrates was assessed in the presence of both proteasome and isopeptidases inhibitors (MG-132 and Ubal) followed by protein separation by SDS-PAGE. The assay was performed in either the absence or presence of an ATP-generating buffer. The protein-incorporated 125I-ubiquitin was visualized by autoradiography. The quantification of each band was normalized relative to the control (100%). Three independent assays were performed, and the values presented in the graph correspond to the mean±SE. The asterisk indicates a difference at p<0.05. GlucOx represents glucose oxidase.