Figure 1 of
Fernandes, Mol Vis 2006;
Figure 1. Production of H2O2 by glucose oxidase
In medium without cells, the concentrations of H2O2 increased in a concentration- and time-dependent manner. When cells were grown in a serum- and phenol red-free medium (DMEM), H2O2 concentrations reached a constant value after approximately 1 h of incubation. Glucose oxidase (40 mU/ml) generated a concentration of approximately 100 μM of H2O2 in the medium. The rate of degradation of H2O2 was 17.6 nmol min-1. The measurements presented correspond to the mean of duplicates.