Table 2 of
Ascano, Mol Vis 2006;
12:1516-1525.
Table 2. Effect of p44 mutants on photolyzed rhodopsin
The ability of p44 mutants to decrease activity of photolyzed rhodopsin was determined by measuring transducin activation as described in the methods and expressed relative to wild type p44. The final concentration of p44 in reactions with unphosphorylated rhodopsin was 5 μM and 2 μM in reaction with phosphorylated rhodopsin and the final concentration of transducin was between 2.5 and 3.0 μM. The ability of p44 mutants to bind to photolyzed rhodospsin was measured by a centrifugation assay as described in the methods and expressed relative to wild type arrestin. The final p44 concentration was 3 μM and the final concentration of rhodopsin in the reaction was 7 μM.
Ability to decrease activity of photolyzed Ability to bind photolyzed rhodopsin (relative to WT p44) rhodopsin (relative to WT p44) --------------------------------- --------------------------------- Unphosphorylated Phosphorylated Unphosphorylated Phosphorylated p44 Mutants rhodopsin rhodopsin rhodopsin rhodopsin ----------- ---------------- -------------- ---------------- -------------- WT p44 1.00 1.00 1.00 1.00 K15A 1.08 0.60 0.84 0.75 K14A/K15A 1.21 0.79 0.34 0.18 R29A 0.41 0.47 0.33 0.22 K300A 0.60 0.51 0.21 0.20 H301A 0.58 0.64 0.69 0.66 |