Table 1 of
Ascano, Mol Vis 2006;
12:1516-1525.
Table 1. Effect of arrestin mutants on photolyzed rhodopsin
The ability of arrestin mutants to decrease activity of photolyzed rhodopsin was determined by measuring transducin activation as described in the methods and expressed relative to wild type arrestin. The final arrestin concentration in all reactions was 5 μM and the final concentration of transducin was between 2.5 and 3.0 μM. The ability of arrestin mutants to bind to photolyzed rhodospsin was measured by a centrifugation assay as described in the methods and expressed relative to wild type arrestin. The final arrestin concentration was 3 μM and the final concentration of rhodopsin in the reaction was 7 μM.
Ability to decrease activity of photolyzed rhodopsin Ability to bind photolyzed (relative to WT-Arr) rhodopsin (relative to WT-Arr) --------------------------------- ----------------------------------- Unphosphorylated Phosphorylated Unphosphorylated Phosphorylated Arrestin mutants rhodopsin rhodopsin rhodopsin rhodopsin ---------------- ---------------- -------------- ---------------- ---------------- WT 1.00 1.00 1.00 1.00 K15E 0.52 0.19 0.90 0.50 R29E 2.95 0.52 2.53 0.98 K14A/K15A/R29E 2.00 0.65 2.75 0.73 K300A 1.28 0.22 1.24 0.97 K300E 1.23 0.13 0.98 0.80 H301A 1.75 1.08 1.28 1.01 H301E 1.84 0.84 1.83 0.93 K300A/H301A 1.42 0.88 1.32 0.96 |