Table 1 of Ascano, Mol Vis 2006; 12:1516-1525.


Table 1. Effect of arrestin mutants on photolyzed rhodopsin

The ability of arrestin mutants to decrease activity of photolyzed rhodopsin was determined by measuring transducin activation as described in the methods and expressed relative to wild type arrestin. The final arrestin concentration in all reactions was 5 μM and the final concentration of transducin was between 2.5 and 3.0 μM. The ability of arrestin mutants to bind to photolyzed rhodospsin was measured by a centrifugation assay as described in the methods and expressed relative to wild type arrestin. The final arrestin concentration was 3 μM and the final concentration of rhodopsin in the reaction was 7 μM.

                         Ability to decrease
                   activity of photolyzed rhodopsin       Ability to bind photolyzed
                          (relative to WT-Arr)           rhodopsin (relative to WT-Arr)
                   ---------------------------------   -----------------------------------
                   Unphosphorylated   Phosphorylated   Unphosphorylated    Phosphorylated
Arrestin mutants      rhodopsin        rhodopsin          rhodopsin          rhodopsin
----------------   ----------------   --------------   ----------------   ----------------
WT                      1.00              1.00               1.00              1.00
K15E                    0.52              0.19               0.90              0.50
R29E                    2.95              0.52               2.53              0.98
K14A/K15A/R29E          2.00              0.65               2.75              0.73
K300A                   1.28              0.22               1.24              0.97
K300E                   1.23              0.13               0.98              0.80
H301A                   1.75              1.08               1.28              1.01
H301E                   1.84              0.84               1.83              0.93
K300A/H301A             1.42              0.88               1.32              0.96

Ascano, Mol Vis 2006; 12:1516-1525 <http://www.molvis.org/molvis/v12/a174/>
©2006 Molecular Vision <http://www.molvis.org/molvis/>
ISSN 1090-0535