Figure 1. Effects of TA on VEGF₁₆₅ and PEDF mRNA expression in RPE cells

Effect of triamcinolone acetonide (TA) on vascular endotheliel growth factor (VEGF₁₆₅, panel A) and pigmented epithelium derived factor (PEDF, panel B) mRNA expression in RPE cells. TA (0.1 and 1.0 mg/ml) was added to the culture medium. Real-time RT-PCR measurements (mean±SD) indicated a 0.5 fold decrease in levels of VEGF₁₆₅ and a 2.5 fold increase in levels of PEDF. The amplifications showed no significant difference between the two concentration of TA, but the effect was maintained till 12 h by 0.1 mg/ml TA and till 24 h by 1.0 mg/ml TA. *p<0.005, control drug-free growth versus TA-treated cultures by ANOVA.