Figure 7. The effect of siRNA treatment on POS phagocytosis by cultured rat RPE cells

A: MFG-E8 mRNA knockdown by treatment with: (a) control, (b) mock transfection (transfection reagent only), (c) non-targeting siRNA, (d) MFG-E8-specific targeting siRNA for 48 h, (e) 72 h, (f) 96 h. B: POS phagocytosis by cultured RPE cells after treatment with: (a), control, (b) mock transfection, (c) non-targeting siRNA, (d) 48 h, (e) 72 h, (f) 96 h incubation with MFG-E8-specific targeting siRNA. C: Western blot of RIPA extracts of cultured RPE cells after (a) no treatment, (b) 96 h MFG-E8-specific targeting siRNA. D, E: Fluorescence image of cultured RPE cells transfected for 96 h with siGlo-labeled cyclophilin siRNA; Panel E is a brightfield image of D. Original magnification 20x.