Figure 3.

Canine myocilin was cloned and sequenced. The canine myocilin deduced amino acid sequence is compared with human and consensus sequences. Nucleic acids from fresh beagle optic nerve head tissue were cloned and sequenced using the primers listed in Table 2. At the nucleotide level, canine myocilin was 82.3% identical to that of human and 82.7% identical at the amino acid level. Yellow highlights identify those amino acids conserved by all species sequenced to date, although many additional residues are the same in dog and human myocilin. Splice junctions are shown in red, and the N-glycosylation sequon is shown in green.