Figure 5 of
Lyu, Mol Vis 2006;
Cytoskeletal rearrangement in insulin-stimulated THCE cells. THCE cells (2x103) were plated on slide chambers coated with diluted Matrigel matrix and starved of serum and growth factors for 24 h. Cells pretreated with DMSO (A, B) 100 nM wortmannin (C), 1 μM AG1478 (D), or 50 μM GM6001 (E) for 1 h were incubated with vehicle (A) or 5 μg/ml insulin (B-E). After 1 h, cells were fixed and stained with rhodamine-phalloidin. The photograph in B shows numerous stress fibers and little submembranous cortical actin (arrowhead). Scale bar, 10 μm.