Figure 5.

Function analysis of selected A10 against survivin in vivo. Nude mice bearing an orthotopic transplant of HCC for 3 days received intravenous oligonucleotides as described in Methods. A: Tumor weight as a function of treatment. Tumor weight was determined by electrobalance. All of the data are represented as the mean±SD of 8 mice. The asterisk indicates a p<0.01 compared with saline treatment. B: Analysis of survivin protein in tumor tissue. Equal amounts of protein from tumor lysates was immunoblotted as described in Methods. A representative sample of tumors in each group treated with saline, A10, or sense is shown, and the experiment was repeated three times with similar results. The number under each band is expressed as a percentage of saline treatment, normalized by the corresponding β-actin level.