Figure 8. Immunohistochemical localization of cholesteryl ester transfer protein in monkey retina

The vibrotome sections from monkey retina were processed for immunhistochemistry and imaged by fluorescent confocal microscopy (see Materials and Methods). Nuclei were stained with DAPI (blue) and immunoreactivity was detected using a Cy5 conjugated secondary antibodies (red). A: cholesteryl ester transfer protein (CETP) was localized using anti-human CETP (synthetic C-terminus peptide) rabbit polyclonal antibody (BioVision Research Products, Mountain View, CA) at 1:50 dilution. B: No primary antibody control. C: CETP immunoreactivity at higher magnification focusing on the photoreceptors and retinal pigment epithelium/choriocapillaris regions. D: CETP immunoreactivity at higher magnification focusing on the outer plexiform layer region. Images C and D are shown with the green channel to take advantage of the retinal autofluorescence and provide better structural definition. Capillaries in D were stained with isolectin IB4 (green). Scale bars were included with each image.