Figure 5. Immunohistochemical localization of SR-BII in monkey retina

The vibrotome sections from monkey retina were processed for immunhistochemistry and imaged by fluorescent confocal microscopy (see Materials and Methods). Nuclei were stained with DAPI (blue) and immunoreactivity was detected using a Cy5 conjugated secondary antibodies (red). A: SR-BII immunoreactivity detected with anti SR-BII peptide CLLEDSLSGQPTSAMA (cross-reacts with many species) rabbit polyclonal antibody (Abcam Inc.) at 1:50 dilution. B: No primary antibody control. C: SR-BII immunoreactivity at higher magnification focusing on the photoreceptors and retinal pigment epithelium/choriocapillaris regions. D: Cross section of photoreceptor region near connecting cilium region. Images C and D are shown with the green channel to take advantage of the retinal autofluorescence and provide better structural definition. Capillaries in C were stained with isolectin IB4 (green). Scale bars were included with each image.