Figure 3 of
Tserentsoodol, Mol Vis 2006;
Figure 3. Immunohistochemical localization of apoA1 in monkey retina
The vibrotome sections from monkey retina were processed for immunhistochemistry and imaged by fluorescent confocal microscopy (see Materials and Methods). Nuclei were stained with DAPI (blue) and immunoreactivity was detected using a Cy5 conjugated secondary antibodies (red). A: ApoA1 immunoreactivity detected with anti-human apoA1 rabbit polyclonal antibody (Abcam Inc.) at 1:50 dilution. B: Control with no primary antibody. C: ApoA1 immunoreactivity at higher magnification focusing on the photoreceptors and retinal pigment epithelium/choriocapillaris regions, arrow points to the inner segments of the rod photoreceptors. D: Higher magnification of the ganglion cell layer. Images C and D are shown with the green channel to take advantage of the retinal autofluorescence and provide better structural definition. Capillaries in D were stained with isolectin IB4 (green). Scale bars were included with each image.