Figure 2. Immunohistochemical localization of ABCA1 in monkey retina

The vibrotome sections from monkey retina were processed for immunhistochemistry and imaged by fluorescent confocal microscopy (see Materials and Methods). Nuclei were stained with DAPI (blue) and immunoreactivity was detected using a Cy5 conjugated secondary antibodies (red). A: ABCA1 immunoreactivity in the macular (fovea) region of the retina was detected using anti-human ABCA1 rabbit polyclonal antibody (Abcam Inc.) at 1:500 dilution. B: No primary antibody control image of the macula. C: ABCA1 immunoreactivity at higher magnification focusing on the photoreceptors and macular retinal pigment epithelium. D: Low magnification image of the immunoreactivity in the peripheral retina. Images C and D are shown with the green channel to take advantage of the retinal autofluorescence and provide better structural definition. Capillaries in D were stained with isolectin IB4 (green). Scale bars were included with each image.