Table 1 of
Riazuddin, Mol Vis 2006;
12:1283-1291.Table 1 of
Riazuddin, Mol Vis 2006;
12:1283-1291.
Table 1. List of primers for sequencing PDE6A gene
Conditions for PCR amplifications are described in Methods. Annealing temperature for each primer pair is given.
Annealing
temperature
Exon Forward primer Reverse primer (°C)
-------- ------------------------- -------------------- -----------
Promoter TGGACAGAGAAGCCAAACAA TTCTCCACCTCCTCTGCTGT 65
1a CCAGACTGGACTTGTTGCAG GAACAGGCTCATGCGGTCT 65
1b TGGAGGAGAGCGAAATCATC ACCTGTACCCCAGAACTCCA 65
2 CCGTTCCACTGTTCTTGCTC GCAAAGTTCAGGGGACTTCA 65
3 GCCAGAGGATGGATTTCTTC TAGGCACCTTCATTCCCATC 65
4 TTGTTGTTATTCTCCAGCTAAGTG TTGAATGTGTGCCAAGACTC 65
5 GACTCATGGAGGTGGGACAT AGACAACCCAACGCAAAGAC 65
6 AGATCAAGCCATTGCACTCC TTGCCCAATTCCAGAATCAC 65
7 TGTAAGCAGGTGCTGAGAGC TCTTTCTTCCACGTGATCCA 65
8 CCTTGGACAAGAACATGGTG CAGCAGAGTGGGTGGATTCT 65
9 TATCATCGTTGCCTCTGTGG TGTGATAGCGCAGTGACACC 65
10 GGCAGCACACAGCTTATCAA ACAGTGCACAAACCCATGC 65
11 GTTGCAAGGACTTTGGAGGA ATGCTTTGCAAGGAGAAACC 65
12 TCTGATCCTTCCAGCAGACC CACAGAGGAACAGCGTGTCT 65
13 GGCCATGCCTTCTTCATATT CAACGCTGTTGCTACCATGT 65
14 CTCCTTACACCCGCCTTTTC CCACAAGACTTCCCTGTTGG 65
15 TCACTTGTGGAGAAGGCTGA GCCAATGGGAAGAATGCTC 65
16 CCATTGGTAGGTGGGTGACT CCTGGGCAACAGAGTGAGAT 65
17 GCCAATGTTAGCAGCTCAGG GCAAGAGCTGTCAGTGCATC 65
18 GGGTGGAGAAAGGTGAGAGA AGTCCAAGCCTCATGACCTG 69
19 AGCAGGGGTAGGGGATTG CTCCATCATGGCGAGGTC 65
20 TGCTTCATAGATAGGGTAGGTTTC CTGGTCACCTGCTAGGGTTT 65
21 GCTACTCCGAAGCAGCTCAT CACACACAGAATGGGGACAG 65
22 GTCAAAGGGGAAGCCCACT GGTCTTCCACTGGCTTGAGT 61
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