Table 6. Opsin mRNAs in normal and glaucoma human eyes

In situ hybridization using each of the three opsin probes was performed on blocks of tissue harvested from the mid-peripheral retina of human donor eyes. Donors in this study had no history of ocular disease (human control represents HC) or a clinical record of being diagnosed with glaucoma (GL). Sections for each specimen were taken en face as described in Methods, and then scored for staining intensity using the following ratings: 0 represents no stain; 1 represents light staining; 2 represents moderate staining; 3 represents marked staining. For comparison, the visual field results of individuals that were diagnosed with glaucoma (if available) were also recorded.

                                        Visual
                                         field
Subject   Age   Rod   Red/Green   Blue   defect
-------   ---   ---   ---------   ----   ------
Normal Eyes

HC 17     69     3       3         3
HC 26     69     2       2         NT
HC 32     73     3       3         3
HC 49     57     3       3         3

Glaucoma Eyes

GL 13     66     3       3         3       No
GL 26     68     3       1         2       NA
GL 46     71     3       0         0       Yes
GL 49     78     3       1         1       Yes
GL 55     73     3       1         1       No
GL 60     78     3       0        NT       Yes
GL 66     83     3       3         3       Yes