Figure 4. Western blot analysis of the Egr1 protein

A 55-kD Egr1 protein appears in wildtype (WT) embryos at 24, 48, and 72 h postfertilization (hpf), whereas the Grade-3 Egr1 morphants (Mo) do not exhibit this at the same periods. After Egr1 mRNA rescue, Egr1 protein appeared in the 48-hpf rescued morphants (ReMo). The leftmost lane represents the molecular weight marker (M).