Figure 7.

Deslorelin and transferrin functionalizations enhance nanoparticle transport (A, C) and uptake (B, D) in isolated corneas and conjunctivas determined using an Ussing chamber setup. Nanoparticles (NP, deslorelin-NP and transferrin-NP) were exposed to isolated bovine cornea and conjunctiva mounted in Ussing chambers. Cumulative transport or uptake at 4 h is presented. Experiments were conducted at 37 °C as well as 4 °C. The data are expressed as mean±SD for n=4. The asterisk indicates a p<0.05 compared to corresponding particles at 37 °C, the sharp (hash mark) indicates a p<0.05 compared to NP group at 37 °C, the double sharp indicates a p<0.05 compared to deslorelin-NP at 37 °C, and the double asterisk indicates a p<0.05 compared to corresponding data for corneal uptake.