Table 1 of
Sakamoto, Mol Vis 2006;
12:117-124.Table 1 of
Sakamoto, Mol Vis 2006;
12:117-124.
Table 1. Real-time PCR primers
Primers were designed using Primer3 on the indicated GenBank sequences. The length of the PCR product for each primer pair and the cDNA sequence number for each primer are indicated. Each primer pair yielded a single product, as determined by agarose gel electrophoresis and melt-curve analyses.
GenBank
accession Size Nucleotide
Name number (bp) Sequence (5'-3') number
-------------------------- --------- ---- ----------------------- ----------
AANAT U38306 431 F: TCACTGAGCTGCAATGTTCC 672- 691
R: GATGTCAGCCTGGCCTAGAG 1083-1102
Rhodopsin Z46957 191 F: GCAGTGTTCATGTGGGATTG 632- 651
R: CTGCCTTCTGAGTGGTAGCC 803- 822
swl opsin AF051163 282 F: GTACCACATTGCTCCCGTCT 69- 80
R: AGACCTGCTACAGAGCCCAA 331- 350
mwl opsin AF031528 326 F: TCATTTCCTGGGAGAGATGG 296- 315
R: TTTCTGTTGCTTTGCCACTG 602- 621
Glyceraldehyde-3-phosphate X02231 142 F: AGACAGCCGCATCTTCTTGT 24- 43
dehydrogenase (gapdh) R: TGATGGCAACAATGTCCACT 146- 165
Tyrosine hydroxylase (th) M10244 247 F: CAGGGCTGCTGTCTTCCTAC 1183-1202
R: GGGCTGTCCAGTACGTCAAT 1410-1429
Per1 AB092976 164 F: ACACCCAGAAGGAAGAGCAA 2354-2373
R: GCGAGAAGCCTTTGCTTTAG 2498-2517
Per2 AB016532 195 F: GAGAGAGGAACAGGGCTTCC 2285-2304
R: TTGACACGSTTGGACTTCAG 2460-2479
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