Figure 1. RS1 message and retinoschisin protein in the rat pineal

A: Northern hybridization for RS1 mRNA from retina (lane 1) and pineal gland (lane 2) was probed with digoxigenin-labeled cRNA probe, with β-actin as control (middle panel). Two bands corresponding to 28S and 18S ribosomal RNA are seen on the ethidium bromide stained agarose gel before transferring to the membranes (lower panel). B: Western blot immunolabeling of retinal (lane 1) and pineal (lane 2) fractions with rabbit anti-RS antibody (upper band) and mouse anti β-actin antibody as the loading control (lower band).