Figure 4. Analysis of the iontophoresis effect duration on the oligonucleotides delivery to the outer nuclear layer

Cathodal saline iontophoresis was performed immediately, 1 h, 3 h, or 6 h before the intravitreal injection of labeled oligonucleotides (ODNs) in PN7 rd1/rd1 mice. One hour after the injection, the penetration of ODNs in outer nuclear layer (ONL) was quantified. The relative fluorescent intensities in ONL were represented by histograms expressed as means±SD (vertical bars).