Figure 4. Mutation analysis of FZD4 gene in family 2

A: Pedigree of the FEVR family. The phenotype-genotype is not known for the individual marked with asterisk. B: The exon 2 PCR product of FZD4 showed an extra band (arrow) on the 10% polyacrylamide gel. C: Sequencing chromatogram of the normal III: 13(left) and affected III: 7(right) DNA revealed the T>C heterozygous change marked by arrowhead that replaces aminoacid Cysteine by Arginine. (C204R).