Figure 3 of Johnson, Mol Vis 2006; 12:1057-1066.


Figure 3.

Quantification of scleral PRELP and decorin by competitive PCR analysis. Decreasing amounts of decorin and PRELP internal standard were added to the scleral cDNA prepared from RNA of a 35-year-old human sclera. Absolute levels of decorin and PRELP in total scleral RNA were determined at the point of cross over between the amplification of decorin (A) or PRELP (B) and the competitive internal standard DNA. At point 0 on the y-axis (C), the concentration of decorin (A) and PRELP (B) equal the anti-log of the internal standard (plotted on the x-axis). From this comparison it was determined that 0.02 pg of decorin and 0.11 pg of PRELP mRNA were present in the scleral RNA pool.

(63 K)
(23 K)

Johnson, Mol Vis 2006; 12:1057-1066 <http://www.molvis.org/molvis/v12/a119/>
©2006 Molecular Vision <http://www.molvis.org/molvis/>
ISSN 1090-0535