Table 3 of
Sharif, Mol Vis 2006;
12:1040-1047.Table 3 of
Sharif, Mol Vis 2006;
12:1040-1047.
Table 3.
Amplification and confirmation of human 5HT receptor mRNAs. The data shown were used to confirm the identity of the 5HT receptor mRNA being detected in human ocular tissues relative to the positive control tissues (human brain and lung).
Endonuclease
PCR enzyme used
Positive product for PCR Endonuclease
5HT control length product digestion
receptor tissue (bp) verification products (bp)
-------- -------- ------- ------------ -------------
2A Brain 277 Bcl1 193, 85
2B Lung 432 HaeIII 348, 84
2C Brain 288 HaeIII 38, 150, 100
3 Brain 393 Aci1 126, 267
4 Brain 398 Alu1 310, 88
5 Brain 486 Alu1 38, 60, 388
6 Brain 198 Pst1 73, 125
7 Brain 772 Bcl1 312, 171, 289
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