Table 1 of
Kim, Mol Vis 2006;
12:1016-1021.Table 1 of
Kim, Mol Vis 2006;
12:1016-1021.
Table 1.
Primers for polymerase chain reaction and single nucleotide extension assay in endothelin-1 (EDN1), endothelin receptor type A (EDNRA), and endothelin receptor type B (EDNRB) polymorphisms.
Annealing
temperature
SNP Direction Primer sequence (°C)
---------------- --------- -------------------------------------- -----------
EDN1:c.-131dupA Forward GGCAGAGAGCTGTCCAAGTC 55
(exon 1) Reverse CCCAAAGGAAAACGAAGAAA
Snapshot TCAGCCCAAGTGCCCTTT
EDN1:c.594G>T Forward CAGGTTTTGTTTGTGCCAGA 53
(exon 5) Reverse GGTCACATAACGCTCTCTGGAAAG
EDNRA:c.-231G>A Forward GGAGAGGCTTCATCCATCC 53
(exon 1) Reverse CCCAGTCCCCGATAAGAAAG
Snapshot gactgactgTTCCCCGGCTTCAGAAAAC
EDNRA:c.*70C>G Forward AGTCTGTTCCTTCCCCCAGT 55
(exon 8) Reverse TCGTAGATGTTGTGGGTGGA
EDNRA:c.*1222C>T Forward TTTTGAAGTGGCCAGATGAG 55
(exon 8) Reverse AAATGCCCAGCAAAAGTCAC
Snapshot gactgactgacCAGAACTTACGATTCTTCACTT
EDNRB:c.831A>G Forward GAAGATAATCATTCCCTGATGAA 55
(exon 4) Reverse CAATCTGCATGCCACTTTTC
Snapshot gactgactgactgactGCAAGCAGAAATAGAAACTGAA
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