Table 1 of
Hanna, Mol Vis 2006;
12:961-976.Table 1 of
Hanna, Mol Vis 2006;
12:961-976.
Table 1. Primer sets against human sequences used in fresh-tissue PCR
"Accession number" represents GenBank accession number for the corresponding human gene. "Location" represents position of initial nucleotide for forward and reverse primer pairs. "Human sequence" represents sequence for design of forward and reverse primers. "Size" represents number of base pairs in expected PCR product.
Accession Size
Gene number Location Human sequence (5'-3') (bp)
-------- --------- -------- --------------------------- ----
GluR1 NM_000827 1870 F: AGACAACCAGTGACCAGTCCAATG 502
2371 R: GAATCCAAGTTACCTCCCACCTTC
GluR2 NM_000826 1812 F: TTTTTGCCTACATTGGGGTCAG 503
2314 R: TTTGGACTTCCGCACTCTAGCCAC
GluR3 NM_000828 1967 F: TTGGAGTCAGCGTAGTTCTTTTCC 501
2467 R: TTCCCTTGGACTTTCGCACTCG
GluR4 NM_000829 1723 F: ACAGAAGAGCCAGAGGACGGAAAG 500
2222 R: TCACATGGCTTTCGCTGCTC
GluR5 NM_000830 1776 F: CCACCATTCTGGAAGAACCCTATG 495
2270 R: TGTTTTCCACCACGTCTGAGTCAG
GluR6 NM_021956 2260 F: CAGATTGGCGGCCTTATAGACTC 456
2715 R: TTCTTTACCTGGCAACCTTCTGTC
GluR7 NM_000831 2282 F: AGATTGGGGGACTCATTGACTC 479
2760 R: TAAGGATGTGCTGCAGGCCATG
KA1 NM_014619 2041 F: AATTCCCGCTACCAGACCTACC 484
2524 R: AGACGGACACCTCAGTTGCTTC
KA2 NM_002088 1287 F: GTGGTCACAACCATCCTGGAGAAC 485
1771 R: AGGCATGGGTGTGGGTTATAC
mGluR1 NM_000838 1824 F: AGGGCCAGATTAAGGTTATACGG 493
2316 R: CAGATCTTCTTCTTGCTGCCAGC
mGluR2 NM_000839 1845 F: CTCTGCTACTGCATGACCTTCATC 489
2333 R: CAGGAATGCCAGCCAGATGATG
mGluR3 NM_000840 1703 F: GTCACTGGGCAGAAACCTTATCG 513
2215 R: AGATAGCGAAGGAACTCCCCAG
mGluR4 NM_000841 1983 F: GTGGTGATCACCTTTGTGCGCTAC 486
2468 R: TGTGACCATGAGCAGCATACTG
mGluR5 NM_000842 3738 F: CCCCGTGTTCACACACACACAATG 520
4457 R: GCCTCCGTAAATACTGGACCCAAG
mGluR6 NM_000843 2675 F: CGGCATGCTCTACGTACCCAAAAC 485
3159 R: AATCTCCCGCAAACCAGGCAAAGC
mGluR7 NM_000844 2281 F: CACTTTCATCCGCTACAATGACAC 483
2763 R: CCGAGTCTTGATGGCATACACAG
mGluR8 NM_000845 1450 F: GGAGATGCTCCTGGACGTTATG 486
1935 R: TAGGAGCACGTAACTAAGTTCGCG
EAAT1
(GLAST) NM_004172 819 F: TAAACAATGTGTCTGAGGCCATGG 207
1025 R: CTACCAGTCTCATGATGGCTTCG
EAAT2
(GLT-1) NM_004171 1256 F: TTCATTGCTTTTGGCATCGC 191
1446 R: TGCCTAGCAACCACTTCTAAGTCC
EAAT3
(EAAC-1) NM_004170 808 F: ATTCAGATGGCATAAACGTCCTGG 188
995 R: CCGAGCAATCAGGAACAAAATACC
EAAT4 NM_005071 706 F: GAACCAGCTTCCTGGAAAATGTC 202
907 R: TTGAGGCTGTCGAAGAAGTCCCTG
EAAT5 NM_006671 877 F: TCAGCTTCTGCCAGTGCCTCAATG 208
1084 R: TAGAGCAGGGGCAGGATAAAGAGC
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