Figure 7. PCR fragment purification and sequencing for macaque retina illustrated for GluR1

A: Electrophoresis separation of three PCR products generated with independent primer sets against overlapping fragments of GluR1; samples were separated by empty lanes to prevent spillover. B: Comparison of a single GluR1 PCR product against a low DNA mass ladder for determining quantity of product for sequencing. C: Assembly of GluR1 fragments to generate the full-length coding region from a total of 3237 sequenced nucleotides. Primers were designed to produce an overlap of at least 100 base pairs for each PCR product.