Figure 4. Locally-applied steroid effects on CAR mice

Mice were immunized with 50 μg of R64 recoverin-derived peptide three times on days 0, 6, and 20 after the first immunization. Anti-CTLA4 antibody was given intraperitoneally to mice on days 0, 3, and 6 after the first immunization, at a concentration of 100 μg/mouse. On days 7 and 21 after first peptide immunization, steroid was given subconjunctively at dose of 50 mg/kg/body weight. A: Humoral response. Immunoblot of recombinant mouse-recoverin probed with sera from immunized mice at 1:400 dilution. Three independent experiments yielded similar results. B: Histological assessment. On day 35 after immunization, eyes were enucleated and fixed for 1 h in 4% phosphate buffered glutaraldehyde and transferred into 10% phosphate buffered formaldehyde until processing. Tissues were stained with hematoxylin-eosin technique.