Table 4 of Cai, Mol Vis 2006; 12:1-14.


Table 4. Genes within adult human RPE (ahRPE) upregulated genes >three fold compared to ARPE19

Data were generated from Affymetrix U95Av2 DNA microarray analysis of four ahRPE and five ARPE19 samples. Numbers shown in ahRPE and ARPE19 columns are the mean values of DNA microarray chip densitometry scan signals. Numbers shown in ahRPE/ARPE19 column are fold-changes comparing ahRPE gene expression level with ARPE19. Numbers in p value column are from student t-test. Asterisk indicates genes within 2,500 most abundant gene list (data not shown).

                                                                      ARPE19/
 Probe ID          Gene title            Symbol     ahRPE    ARPE19    ahRPE    p value     Gene functions
-----------   ----------------------   ----------   ------   ------   -------   --------   -----------------
36533_at*     prostaglandin I2         PTGIS        1254.8     71.2    17.6     0.000023   electron
              (prostacyclin)                                                               transport; lipid
              synthase                                                                     metabolism

41294_at*     keratin 7                KRT7         4157.1    239.2    17.4     0.000250   cytoskeleton
                                                                                           organization and
                                                                                           biogenesis

34265_at*     secretory granule,       SGNE1         305.6     45.3     6.7     0.000000   protein folding;
              neuroendocrine protein                                                       intracellular
              1 (7B2 protein)                                                              protein transport

32434_at      myristoylated            MARCKS        328.3     50.8     6.5     0.000000   cell motility
              alanine-rich protein
              kinase C substrate

38957_at      doublecortin and CaM     DCAMKL1       260.0     43.4     6.0     0.001277   protein
              kinase-like 1                                                                phosphorylation;
                                                                                           endosome
                                                                                           transport

41505_r_at    v-maf                    MAF           133.3     23.6     5.7     0.001446   regulation of
              musculoaponeurotic                                                           transcription
              fibrosarcoma oncogene
              homolog

39927_at      Rho GTPase activating    ARHGAP5       120.4     21.4     5.6     0.000301   cell adhesion
              protein 5

40367_at      bone morphogenetic       BMP2          245.6     49.9     4.9     0.000051   skeletal
              protein 2                                                                    development;
                                                                                           cell-cell
                                                                                           signaling

40455_at*     KIAA0830 protein         KIAA0830      609.6    125.1     4.9     0.000021   nucleic acid
                                                                                           binding;
                                                                                           endonuclease
                                                                                           activity

865_at*       ribosomal protein S6     RPS6KA3       936.4    194.0     4.8     0.000269   skeletal
              kinase, 90 kDa,                                                              development;
              polypeptide 3                                                                protein
                                                                                           phosphorylation

40035_at*     kallikrein 11            KLK11        1023.1    213.3     4.8     0.000000   proteolysis and
                                                                                           peptidolysis

40493_at*     CD44 antigen             CD44         2812.0    604.9     4.6     0.000027   cell adhesion;
                                                                                           cell-matrix
                                                                                           adhesion

33103_s_at    adducin 3 (γ)            ADD3          115.8     25.1     4.6     0.000006   calmodulin
                                                                                           binding

38634_at*     retinol binding          RBP1         1152.3    250.5     4.6     0.000012   vitamin A
              protein 1, cellular                                                          metabolism;
                                                                                           transport

33236_at*     retinoic acid receptor   RARRES3       406.3     90.8     4.5     0.000080   growth regulatory
              responder (tazarotene                                                        protein; tumor
              induced) 3                                                                   suppressor

31720_s_at*   fibronectin 1            FN1          1496.7    341.1     4.4     0.000050   cell adhesion;
                                                                                           cell migration

33849_at      pre-B-cell colony        PBEF1         150.5     34.6     4.4     0.003907   signal
              enhancing factor 1                                                           transduction;
                                                                                           cell
                                                                                           proliferation
                                                                                           regulation

38138_at*     S100 calcium binding     S100A11      3690.0    848.9     4.3     0.000001   negative
              protein A11                                                                  regulation of
              (calgizzarin)                                                                cell
                                                                                           proliferation

34642_at*     tyrosine                 YWHAZ        2301.6    544.5     4.2     0.000003   protein domain
              3-monooxygenase                                                              specific binding
              activation protein

266_s_at      CD24 antigen (small      CD24          129.5     32.0     4.0     0.005621   immune response
              cell lung carcinoma
              cluster 4 antigen)

40227_at*     discoidin, CUB and       DCBLD2       1007.5    260.5     3.9     0.000037   cell adhesion;
              LCCL domain containing                                                       wound healing
              2

1456_s_at     interferon,              IFI16         118.2     31.1     3.8     0.000007   immune response;
              γ-inducible                                                                  cell
              protein 16                                                                   proliferation

32533_s_at*   vesicle-associated       VAMP5         577.1    155.0     3.7     0.000002   myogenesis;
              membrane protein 5                                                           vesicle-mediated
              (myobrevin)                                                                  transport

40500_at      NDRG family member 4     NDRG4         290.6     78.8     3.7     0.000398   response to
                                                                                           stress; cell
                                                                                           differentiation

1991_s_at*    mitogen-activated        MAPKAPK3     1195.9    329.0     3.6     0.000009   protein
              protein                                                                      phosphorylation;
              kinase-activated                                                             response to
              protein kinase 3                                                             stress

35726_at*     S100 calcium binding     S100A2       1584.2    437.1     3.6     0.000071   calcium ion
              protein A2                                                                   binding

36262_at*     glucosamine              GNS          1089.7    303.0     3.6     0.000969   glycosaminoglycan
              (N-acetyl)-6-sulfatase                                                       catabolism;
              (Sanfilippo disease                                                          metabolism
              IIID)

33863_at*     hypoxia upregulated 1    HYOU1        4796.3   1336.2     3.6     0.000001   protein folding;
                                                                                           response to
                                                                                           stress

672_at*       serine (or cysteine)     SERPINE1     2268.9    676.6     3.4     0.000211   blood coagulation
              proteinase inhibitor

35769_at*     G protein-coupled        GPR56        1484.9    445.4     3.3     0.000580   neuropeptide
              receptor 56                                                                  signaling pathway;
                                                                                           metabolism

37628_at*     monoamine oxidase B      MAOB          289.5     87.3     3.3     0.000175   electron
                                                                                           transport

37319_at*     insulin-like growth      IGFBP3       1438.0    437.2     3.3     0.008582   cell growth and
              factor binding protein                                                       apoptosis
              3                                                                            regulation

34886_at*     radixin                  RDX           402.1    123.6     3.3     0.000024   cytoskeletal
                                                                                           anchoring

38500_at*     CGI-109 protein          CGI-109       611.8    190.3     3.2     0.000012   intracellular
                                                                                           protein transport

33339_g_at    signal transducer and    STAT1         111.9     35.0     3.2     0.000024   regulation of
              activator of                                                                 cell cycle;
              transcription 1,                                                             caspase
              91 kDa                                                                       activation

39373_at*     fatty acid desaturase    FADS1         478.7    150.3     3.2     0.001098   fatty acid
              1                                                                            biosynthesis;
                                                                                           cell-cell
                                                                                           signaling

37391_at*     cathepsin L              CTSL         3573.0   1126.1     3.2     0.000572   proteolysis and
                                                                                           peptidolysis

1034_at*      tissue inhibitor of      TIMP3        1293.7    412.0     3.1     0.000456   visual
              metalloproteinase 3                                                          perception;
                                                                                           induction of
                                                                                           apoptosis

37405_at*     selenium binding         SELENBP1      995.0    317.0     3.1     0.000836   selenium binding
              protein 1

160020_at*    matrix                   MMP14        2332.0    748.7     3.1     0.000168   proteolysis and
              metalloproteinase 14                                                         peptidolysis
              (membrane-inserted)

35992_at*     musculin (activated      MSC           576.5    186.0     3.1     0.000157   regulation of
              B-cell factor-1)                                                             transcription

31783_at*     renin binding protein    RENBP         321.7    103.8     3.1     0.000111   mannose
                                                                                           metabolism

40848_g_at    flavoprotein             MICAL2        263.6     85.4     3.1     0.000186   electron
              oxidoreductase MICAL2                                                        transport;
                                                                                           metabolism

33989_f_at*   testis enhanced gene     TEGT         3358.8   1092.8     3.1     0.000171   negative
              transcript (BAX                                                              regulation of
              inhibitor 1)                                                                 apoptosis

40913_at*     ATPase, Ca++             ATP2B4        734.8    239.6     3.1     0.000002   calcium ion
              transporting, plasma                                                         transport;
              membrane 4                                                                   metabolism

38837_at      hypothetical protein     DJ971N18.2    174.9     57.2     3.1     0.000004   electron
              DJ971N18.2                                                                   transport

33372_at*     RAB31, member RAS        RAB31         830.0    271.3     3.1     0.000467   small GTPase
              oncogene family                                                              mediated signal
                                                                                           transduction

2092_s_at     secreted                 SPP1           93.7     30.7     3.1     0.000096   anti-apoptosis;
              phosphoprotein 1                                                             cell-matrix
                                                                                           adhesion

41739_s_at    caldesmon 1              CALD1         224.9     73.8     3.0     0.000160   muscle
                                                                                           contraction;
                                                                                           muscle
                                                                                           development

40433_at*     glucosamine              GNS           575.3    191.0     3.0     0.000111   glycosaminoglycan
              (N-acetyl)-6-sulfatase                                                       catabolism;
              (Sanfilippo disease                                                          metabolism
              IIID)

Cai, Mol Vis 2006; 12:1-14 <http://www.molvis.org/molvis/v12/a1/>
©2006 Molecular Vision <http://www.molvis.org/molvis/>
ISSN 1090-0535