Table 1 of
Kirwan, Mol Vis 2005;
Table 1. Applied Biosystems primer and probe assay identifiers (Assay-on-DemandTM)
This table lists the Applied Biosystems primer and probe identifier numbers and the Unigene ID for the target genes that were used for validation of the microarray analysis. Each identifer refers to a pre-developed assay reagent (PDAR) which contain the necessary PCR reagents including pre-designed forward and reverse primers for the target and control genes, pre-optimized in one solution for use with TaqManTM universal PCR master mix. PDARs were obtained from Applied Biosystems by supplying the retrieved GenBank FASTA mRNA sequence for each target gene.
Primer & Gene Unigene ID probe assay ID ------------------- ---------- -------------- Elastin Hs.252418 Hs00355783_m1 BMP-7 Hs.170195 Hs00233477_m1 VEGF Hs.73793 Hs00173626_m1 Biglycan Hs.821 Hs00156076_m1 Thrombomodulin Hs.2030 Hs00264920_s1 EMMPRIN Hs.501293 Hs00174305_m1 Versican Hs.43488 Hs00171642_m1 Collagen VI alpha I Hs.415997 Hs00242448_m1 TGF-B2 Hs.169300 Hs00236092_m1