Figure 1. Cell culture experimental design

Four separate passages of lamina cribrosa (LC) cells (n=4) were generated from three human donors. Three passages of cells from donors 1 and 2 were used for the microarray analysis (pooled; n=3) and the fourth passage of cells from donor 3 was used for the real time PCR validation. All experiments were conducted on LC cells between passages 4 and 6.