Table 1 of
Cameron, Mol Vis 2005;
11:775-791.Table 1 of
Cameron, Mol Vis 2005;
11:775-791.
Table 1. Primer information for RT- and real-time qPCR analyses
For each targeted gene, the table provides infomation about the attendant forward (F) and reverse (R) primer sequences, length, melt temperature of the PCR-amplified product, and optimized annealing temperature (Ann.) empirically determined for the real-time qPCR experiments. Melting temperatures are given as mean±standard deviation.
PCR product Temperature (°C)
size (base ----------------
Target Primer sequences (5'-3') pairs) Ann. Melting
-------------- ------------------------- ----------- ---- ---------
rhodopsin F: ACAACCCCTGCATCTACATC 360 65 91.8±0.3
(rhod) R: CTGTCCTCTCTCAAAACTGTC
phosphoglucose F: TTCATTCCCACGACTCCTCC 225 69 86.6±0.1
isomerase 1 R: TCACACAAACACACACGAAAC
(pgi1)
β-tubulin F: AAACCGCCGTCTGCGATATTCC 322 67 90.3±0.1
R: ACTACCACCTCCCCAAAACACC
plasticin F: AGCATCGCCTCCAAAAAC 213 61 87.7±0.1
R: CCTCCATTTCCCTCATCTGAC
chemokine F: GCGACTTTTTTCACCATTCC 371 64 87.6±0.1
C-X-C motif R: TTTCTCCAGACCCTGTTCC
receptor 4b
(cxcr4b)
growth F: AAAGAGGAGAAAGAGGAGGAG 251 64 89.6±0.2
associated R: CGCAAACCGCAGAAATCAG
protein 43
(GAP43)
cadherin 2 F: TACAGCCACCGTATCCATCC 307 64 89.8±0.1
(cdh2) R: GCACACCATTGTCTGAAGCC
notch2 F: AACCCACACACAATGCCAC 379
R: CCCCCAAAATGAGACTCCTTAC
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