Table 1 of
Cameron, Mol Vis 2005;
11:775-791.
Table 1. Primer information for RT- and real-time qPCR analyses
For each targeted gene, the table provides infomation about the attendant forward (F) and reverse (R) primer sequences, length, melt temperature of the PCR-amplified product, and optimized annealing temperature (Ann.) empirically determined for the real-time qPCR experiments. Melting temperatures are given as mean±standard deviation.
PCR product Temperature (°C) size (base ---------------- Target Primer sequences (5'-3') pairs) Ann. Melting -------------- ------------------------- ----------- ---- --------- rhodopsin F: ACAACCCCTGCATCTACATC 360 65 91.8±0.3 (rhod) R: CTGTCCTCTCTCAAAACTGTC phosphoglucose F: TTCATTCCCACGACTCCTCC 225 69 86.6±0.1 isomerase 1 R: TCACACAAACACACACGAAAC (pgi1) β-tubulin F: AAACCGCCGTCTGCGATATTCC 322 67 90.3±0.1 R: ACTACCACCTCCCCAAAACACC plasticin F: AGCATCGCCTCCAAAAAC 213 61 87.7±0.1 R: CCTCCATTTCCCTCATCTGAC chemokine F: GCGACTTTTTTCACCATTCC 371 64 87.6±0.1 C-X-C motif R: TTTCTCCAGACCCTGTTCC receptor 4b (cxcr4b) growth F: AAAGAGGAGAAAGAGGAGGAG 251 64 89.6±0.2 associated R: CGCAAACCGCAGAAATCAG protein 43 (GAP43) cadherin 2 F: TACAGCCACCGTATCCATCC 307 64 89.8±0.1 (cdh2) R: GCACACCATTGTCTGAAGCC notch2 F: AACCCACACACAATGCCAC 379 R: CCCCCAAAATGAGACTCCTTAC |