Table 1 of Cameron, Mol Vis 2005; 11:775-791.


Table 1. Primer information for RT- and real-time qPCR analyses

For each targeted gene, the table provides infomation about the attendant forward (F) and reverse (R) primer sequences, length, melt temperature of the PCR-amplified product, and optimized annealing temperature (Ann.) empirically determined for the real-time qPCR experiments. Melting temperatures are given as mean±standard deviation.

                                             PCR product   Temperature (°C)
                                             size (base    ----------------
    Target       Primer sequences (5'-3')      pairs)      Ann.    Melting
--------------   -------------------------   -----------   ----   ---------
rhodopsin        F: ACAACCCCTGCATCTACATC         360        65    91.8±0.3
(rhod)           R: CTGTCCTCTCTCAAAACTGTC

phosphoglucose   F: TTCATTCCCACGACTCCTCC         225        69    86.6±0.1
isomerase 1      R: TCACACAAACACACACGAAAC
(pgi1)


β-tubulin        F: AAACCGCCGTCTGCGATATTCC       322        67    90.3±0.1
                 R: ACTACCACCTCCCCAAAACACC

plasticin        F: AGCATCGCCTCCAAAAAC           213        61    87.7±0.1
                 R: CCTCCATTTCCCTCATCTGAC

chemokine        F: GCGACTTTTTTCACCATTCC         371        64    87.6±0.1
C-X-C motif      R: TTTCTCCAGACCCTGTTCC
receptor 4b
(cxcr4b)

growth           F: AAAGAGGAGAAAGAGGAGGAG        251        64    89.6±0.2
associated       R: CGCAAACCGCAGAAATCAG
protein 43
(GAP43)

cadherin 2       F: TACAGCCACCGTATCCATCC         307        64    89.8±0.1
(cdh2)           R: GCACACCATTGTCTGAAGCC

notch2           F: AACCCACACACAATGCCAC          379
                 R: CCCCCAAAATGAGACTCCTTAC

Cameron, Mol Vis 2005; 11:775-791 <http://www.molvis.org/molvis/v11/a93/>
©2005 Molecular Vision <http://www.molvis.org/molvis/>
ISSN 1090-0535