Figure 4 of
Seomun, Mol Vis 2005;
Figure 4. H2O2 induced p21Cip1 accumulation is mediated by JNK and ERK activations
A: HLE B-3 cells were treated for 30 min with specific kinases inhibitors before treatment with 200 μM H2O2. The kinase inhibitors used were 20 μM SB203580 (SB), 20 μM SP600125 (SP), 1 μM Wortmanin (W), 20 μM U0126 (U), and 10 μM GF109203X (GF) which inhibit p38, JNK, PI-3 kinase, MEK, and PKC, respectively. p21Cip1 and actin levels were measured after 16 h of H2O2 treatment. B: HLE B-3 cells were stimulated with 200 μM of H2O2 for the indicated time period. After lysis, 10 μg (for ERK) or 30 μg (for JNK) of total proteins were loaded on 10% PAGE gel. Phosphorylated (p-ERK and p-JNK) or nonphosphorylated levels of JNK and ERK were measured by western blot analysis using specific antibodies. C: FACS analysis showed that SP600125 and U0126 blocked G2/M phase arrest caused by H2O2. The data shown are representative of two to three independent experiments.