Figure 3 of
Seomun, Mol Vis 2005;
Figure 3. H2O2 mediated p21Cip1 and G2/M phase arrest is attenuated by p21Cip1 siRNA
HLE B-3 cells were transfected with 100 nM of p21Cip1 siRNA. After 24 h, the cells were incubated in the absence or presence of 200 μM H2O2 for an additional 24 h, collected, and subjected to both western blot analysis (A) and FACS analysis (B) to confirm the attenuation of p21Cip1 and cell cycle arrest, respectively. The graph shown in A represented the quantification of p21Cip1 expression (performed by scanning densitometry). The bars shown (A, lower) represent the means±SE of three independent expressions and are expressed as the fold induction above control (unstimulated) values.